WHO Publications

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TB CARE I Toolbox

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GLOBAL LABORATORY INITIATIVES Toolbox

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NDWG Newsletters

Emerging Bacterial Pathogens Unit
Laboratory protocols

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        Safety

• Emergency procedure in case of major biohazard incident
  

        Procedures

• Ziehl-Neelsen staining
  
• Auramine staining
  
• Specimen processing for culture
• Preparation of drugs for DST testing
  
• Drug Susceptibility Test (DST)
  
• Identification of Mycobacterium tuberculosis
  
• DNA extraction
  
• Line probe assay
• Maintenance of mycobacterial strains
• Quantferon-TB GIT
• T-SPOT.TB

        Genotyping of Mycobacterium tuberculosis

• Spoligotyping
• MIRU_VNTR

         Gene Xpert MTB/Rif

• Maintenance of GeneXpert
• Troubleshooting GeneXpert

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